Publication year: 2010 Source: Journal of Insect Physiology, In Press, Uncorrected Proof, Available online 21 November 2010 George D., Yocum , Joseph P., Rinehart , Marnie L., Larson A multiplex PCR protocol was developed using five diapause-regulated genes to monitor diapause development of the Colorado potato beetle under field conditions. A total of 870 beetles from the Red River valley of North Dakota and Minnesota, USA, were screened for three consecutive years. Out of the 32 possible expression profiles, eight could be arranged in chronological order of diapause development

The rest is here:
Monitoring diapause development in the Colorado potato beetle, Leptinotarsa decemlineata, under field conditions using molecular biomarkers☆